Fig 1: PAIP1 upregulation attenuated the effect of circ_0005576 silence on CC cell progression. A The transfection efficiency of pcDNA-PAIP1 was determined by qRT-PCR. B–M HeLa and SiHa cells were transfected with si-NC, si-circ_0005576, si-circ_0005576 + pcDNA, or si-circ_0005576 + pcDNA-PAIP1. B–G The cell proliferative capacity was tested by CCK-8 assay, EDU incorporation assay and colony formation assay. H–K The migration and invasion were evaluated by transwell assay. (L and M) The levels of cyclin D1, vimentin, and MMP9 were estimated by western blot. **P < 0.01, ***P < 0.001
Fig 2: Circ_0005576 facilitated PAIP1 expression by sponging miR-1305. A The mRNA level of PAIP1 in CC tissues and normal tissues was measured by qRT-PCR. B The protein level of PAIP1 in CC cells and normal cells was detected by qRT-PCR. C The putative binding sites for miR-1305 and PAIP1 3'UTR were predicted by TargetScanHuman database. D, E The relationship between miR-1305 and PAIP1 was confirmed by dual-luciferase reporter assay. F The overexpression efficiency of miR-1305 was validated by qRT-PCR in HeLa and SiHa cells. G The protein level of PAIP1 was detected in HeLa and SiHa cells transfected with miR-1305 or miR-NC. H The correlation between miR-1305 and PAIP1 was analyzed by Spearman’s correlation analysis. I, J The expression of PAIP1 in HeLa and SiHa cells introduced with si-NC, si-circ_0005576, si-circ_0005576 + anti-NC, or si-circ_0005576 + anti-miR-1305 was examined by western blot, respectively. **P < 0.01, ***P < 0.001
Supplier Page from Abcam for Anti-PAIP1 antibody [EPR13259]